hind limb grip strength  (Columbus Instruments)

Journal: Frontiers in Molecular Neuroscience

Article Title: Disease-modifying rdHSV-CA8* non-opioid analgesic gene therapy treats chronic osteoarthritis pain by activating Kv7 voltage-gated potassium channels

doi: 10.3389/fnmol.2024.1416148

Figure Lengend Snippet: Neuronal DRG Transduction by vHCA8. IHC was performed with NeuN neuronal antigen expression, and V5 antibody to identify exogenous CA8* peptide expression on D14 after vHCA8*; using three different routes of administration including injection into the knee joint (KJ) (A,D,I) ; rear foot pad ( RFP ) (B,E,I) ; and sciatic nerve (SN) (C,F–I) . About 35–50% of ipsilateral lumbar 4–5 DRG neurons were V5-positive after vHCA8*WT treatment. The percentage of SN V5-positive neurons was higher than that in the KJ and RFP groups. Only about 5% of DRG neurons were V5-positive after vHCA8*MT treatment (G) . There was no V5-positive signal from contralateral DRG (H) . ImageJ version 1.54 was used to count stained cells. Groups were compared using IBM SPSS Statistics version 28 to do Student’s t -test, or ANOVA, followed by Fisher’s protected least significant difference (LSD) test, and these data are presented as mean ± SEM. Statistical significance was p < 0.05. Scale = 100 μm.

Article Snippet: Weight-bearing distribution between the left (MIA) and right (contralateral) hind limbs were tested by the Librae Incapacitance Tester (Ugo Basile) in mice after various treatments.

Techniques: Transduction, Expressing, Injection, Staining

Journal: Frontiers in Molecular Neuroscience

Article Title: Disease-modifying rdHSV-CA8* non-opioid analgesic gene therapy treats chronic osteoarthritis pain by activating Kv7 voltage-gated potassium channels

doi: 10.3389/fnmol.2024.1416148

Figure Lengend Snippet: vHCA8*WT reverses MIA-OA-induced decreases in weight-bearing. Male C57BL/6 mice underwent IA KJ injection with 1 mg of monosodium iodoacetate (MIA) on D1 (after Baseline was established) producing hyperalgesia in all mice. Weight-bearing was assessed at about the same time each day as weight (grams) of ipsilateral (treated) limb/weight of ipsilateral limb + weight of contralateral limb (untreated control). On D3 mice were treated with IA KJ injections of either vHCA8*WT at the High-Dose (HD) (1E6 PFU), Mid-Dose (MD) (1E5 PFU) or vHCA8*MT High-Dose (HD) (1E6 PFU) (virus arrow) after weight-bearing assessment. Weight-bearing as measured (grams) dropped starting on D1 after MIA injection in all mice. Weight-bearing increased significantly in mice treated with vHCA8*WT-HD or vHCA8*WT-MD on D27 and D34, respectively, as compared to mice treated with vHCA8*MT-HD. * p < 0.05 for vHCA8*WT-HD vs. vHCA8*MT-HD, # p < 0.05 for vHCA8*WT-MD vs. vHCA8*MT-HD. Data were analyzed for statistical significance between different time points for each group, as compared to Baseline, by one-way analysis of variance (ANOVA) followed by Fisher’s LSD post-hoc test. Repeated measure two-way ANOVA analyses found significant differences in weight distribution between left (OA) and right (contralateral control) hind limbs across all time points [ F (14, 196) = 9.637, p = 3.898E-16]. N = 10 per group.

Article Snippet: Weight-bearing distribution between the left (MIA) and right (contralateral) hind limbs were tested by the Librae Incapacitance Tester (Ugo Basile) in mice after various treatments.

Techniques: Injection, Control, Virus